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crispr guide constructs targeting tet1  (Addgene inc)


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    Structured Review

    Addgene inc crispr guide constructs targeting tet1
    Crispr Guide Constructs Targeting Tet1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lenticrispr+constructs/lentiCRISPR+v2-Blast+(Plasmid+%2383480)/pm42869907-371-0-18
    Average 96 stars, based on 235 article reviews
    crispr guide constructs targeting tet1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Construct:

    Article Title: A NPAS4–NuA4 complex couples synaptic activity to DNA repair
    Article Snippet: .. To generate LentiCRISPR constructs used for testing sBLISS-seq in cultured neurons, the following guide sequences were cloned into LentiCRISPRv2GFP (Addgene, 82416) using the listed primer sequences: (1) Scg2 5′-cggcccgagccctcactca-3′: primer F: 5′-caccgcggcccgagccctcactcag-3′; primer R: 5′-aaacctgagtgagggctcgggccgc-3′; (2) Inhba enhancer 5′-gagcagccactagcgaaccc-3′: primer F: 5′-caccggagcagccactagcgaaccc-3′; primer R: 5′-aaacgggttcgctagtggctgctcc-3′; (3) Bdnf 5′-tgatagtggaaattgcatg-3′: primer F: 5′-caccgtgatagtggaaattgcatgg-3′; primer R: 5′-aaacccatgcaatttccactatcac-3′; (4) Fos 5′-gcgcggtcactgctcgttc-3′: primer F: 5′-caccggcgcggtcactgctcgttcg-3′; primer R: 5′-aaaccgaacgagcagtgaccgcgc-3′. ..

    Article Title: Acid-adapted cancer cells alkalinize their cytoplasm by degrading the acid-loading membrane transporter anion exchanger 2, SLC4A2.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: VACO10MS Human: VACO4A Human: VACO4S Human: VACO5 WBL WBL WBL WBL N/A N/A N/A N/A Human: MIA Pa-Ca-2 Prof. Alessandra Fiorio, University of Lille, France N/A Human PANC-1 Prof. Anna Trauzold, University of Kiel, Germany N/A Experimental models: Organisms/strains Female athymic Nude Crl:NU(NCr)-Foxn1nu mice Charles River N/A Biological samples Matched Pair – Paraffin Tissue Section BioChain T8235090 Oligonucleotides LentiCRISPR sgSLC4A2 AAGAATCTGCGCCCTTGGCG Invitrogen N/A LentiCRISPR sgNDUFS1 TAGAATGTATGCCTACTTGG Invitrogen N/A siGENOME siControl Dharmacon D-001210-01-05 siGENOME siBNIP3 Dharmacon M-004636-01-0005 siGENOME siPEG10 Dharmacon M-032579-01-0005 siGENOME siSLC16A7 Dharmacon M-007409-01-0005 siGENOME siATP6V0A2 Dharmacon M-019950-00-0005 siGENOME siCEACAM6 Dharmacon M-015306-01-0005 siGENOME siPMP22 Dharmacon M-010616-02-0005 siGENOME siCA9 Dharmacon M-005244-02-0005 siGENOME siSLC4A2 Dharmacon M-007582-01-0005 siGENOME siSLC4A3 Dharmacon M-007583-02-0005 siGENOME siSLC26A3 Dharmacon M-007492-00-0005 siGENOME siSLC26A6 Dharmacon M-007494-01-0005 siGENOME siTFEB Dharmacon M-009798-02-0005 siGENOME ATG5 Dharmacon M-004374-04-0005 Recombinant DNA lentiCRISPR v2 Sanjana et al., 201459 Addgene: 52961 lentiCRISPR constructs with gRNA insert listed above In this paper Software and algorithms Fiji ImageJ N/A Gen5 v.10 Biotek N/A MATLAB R2020b Mathworks N/A ..

    Article Title: Relative expression of KLK5 to LEKTI is associated with aggressiveness of oral squamous cell carcinoma
    Article Snippet: Both synthesis of primers and oligos and sequencing of PCR products and clones were performed at the Food and Drug Administration - Center for Biologics Evaluation and Research (CBER) Facility for Biotechnology Resources at National Institutes of Health (Bethesda, MD, USA). .. The generated LentiCRISPR constructs were transfected together with the packaging plasmid psPAX2 (Addgene: 12260), and the envelope plasmid pMD2.G (Addgene: 12259) into HEK293T cells using lipofectamine 3000 as directed by the manufacturer (Thermo Fisher Scientific). ..

    Article Title: Dynamic IL-6R/STAT3 signaling leads to heterogeneity of metabolic phenotype in pancreatic ductal adenocarcinoma cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains 5-alpha Competent E. coli (High Efficiency) New England Biolabs C2987H Premo FUCCI Cell Cycle Sensor (BacMam 2.0) Life Technologies P36238 Recombinant DNA Laconic/pcDNA3.1( ) San Martin et al., 201325 Addgene: 44238 lentiCRISPR v2 Sanjana et al., 201449 Addgene: 52961 lentiCRISPR constructs with gRNA insert listed above This paper N/A Software and algorithms Fiji ImageJ N/A Gen5 v.10 Biotek N/A MATLAB R2020b Mathworks N/A Singscore R package Foroutan et al., 201850 N/A Cell Reports 43, 113612, January 23, 2024 19 ..

    Cell Culture:

    Article Title: A NPAS4–NuA4 complex couples synaptic activity to DNA repair
    Article Snippet: .. To generate LentiCRISPR constructs used for testing sBLISS-seq in cultured neurons, the following guide sequences were cloned into LentiCRISPRv2GFP (Addgene, 82416) using the listed primer sequences: (1) Scg2 5′-cggcccgagccctcactca-3′: primer F: 5′-caccgcggcccgagccctcactcag-3′; primer R: 5′-aaacctgagtgagggctcgggccgc-3′; (2) Inhba enhancer 5′-gagcagccactagcgaaccc-3′: primer F: 5′-caccggagcagccactagcgaaccc-3′; primer R: 5′-aaacgggttcgctagtggctgctcc-3′; (3) Bdnf 5′-tgatagtggaaattgcatg-3′: primer F: 5′-caccgtgatagtggaaattgcatgg-3′; primer R: 5′-aaacccatgcaatttccactatcac-3′; (4) Fos 5′-gcgcggtcactgctcgttc-3′: primer F: 5′-caccggcgcggtcactgctcgttcg-3′; primer R: 5′-aaaccgaacgagcagtgaccgcgc-3′. ..

    Clone Assay:

    Article Title: A NPAS4–NuA4 complex couples synaptic activity to DNA repair
    Article Snippet: .. To generate LentiCRISPR constructs used for testing sBLISS-seq in cultured neurons, the following guide sequences were cloned into LentiCRISPRv2GFP (Addgene, 82416) using the listed primer sequences: (1) Scg2 5′-cggcccgagccctcactca-3′: primer F: 5′-caccgcggcccgagccctcactcag-3′; primer R: 5′-aaacctgagtgagggctcgggccgc-3′; (2) Inhba enhancer 5′-gagcagccactagcgaaccc-3′: primer F: 5′-caccggagcagccactagcgaaccc-3′; primer R: 5′-aaacgggttcgctagtggctgctcc-3′; (3) Bdnf 5′-tgatagtggaaattgcatg-3′: primer F: 5′-caccgtgatagtggaaattgcatgg-3′; primer R: 5′-aaacccatgcaatttccactatcac-3′; (4) Fos 5′-gcgcggtcactgctcgttc-3′: primer F: 5′-caccggcgcggtcactgctcgttcg-3′; primer R: 5′-aaaccgaacgagcagtgaccgcgc-3′. ..

    Recombinant:

    Article Title: Acid-adapted cancer cells alkalinize their cytoplasm by degrading the acid-loading membrane transporter anion exchanger 2, SLC4A2.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: VACO10MS Human: VACO4A Human: VACO4S Human: VACO5 WBL WBL WBL WBL N/A N/A N/A N/A Human: MIA Pa-Ca-2 Prof. Alessandra Fiorio, University of Lille, France N/A Human PANC-1 Prof. Anna Trauzold, University of Kiel, Germany N/A Experimental models: Organisms/strains Female athymic Nude Crl:NU(NCr)-Foxn1nu mice Charles River N/A Biological samples Matched Pair – Paraffin Tissue Section BioChain T8235090 Oligonucleotides LentiCRISPR sgSLC4A2 AAGAATCTGCGCCCTTGGCG Invitrogen N/A LentiCRISPR sgNDUFS1 TAGAATGTATGCCTACTTGG Invitrogen N/A siGENOME siControl Dharmacon D-001210-01-05 siGENOME siBNIP3 Dharmacon M-004636-01-0005 siGENOME siPEG10 Dharmacon M-032579-01-0005 siGENOME siSLC16A7 Dharmacon M-007409-01-0005 siGENOME siATP6V0A2 Dharmacon M-019950-00-0005 siGENOME siCEACAM6 Dharmacon M-015306-01-0005 siGENOME siPMP22 Dharmacon M-010616-02-0005 siGENOME siCA9 Dharmacon M-005244-02-0005 siGENOME siSLC4A2 Dharmacon M-007582-01-0005 siGENOME siSLC4A3 Dharmacon M-007583-02-0005 siGENOME siSLC26A3 Dharmacon M-007492-00-0005 siGENOME siSLC26A6 Dharmacon M-007494-01-0005 siGENOME siTFEB Dharmacon M-009798-02-0005 siGENOME ATG5 Dharmacon M-004374-04-0005 Recombinant DNA lentiCRISPR v2 Sanjana et al., 201459 Addgene: 52961 lentiCRISPR constructs with gRNA insert listed above In this paper Software and algorithms Fiji ImageJ N/A Gen5 v.10 Biotek N/A MATLAB R2020b Mathworks N/A ..

    Article Title: Dynamic IL-6R/STAT3 signaling leads to heterogeneity of metabolic phenotype in pancreatic ductal adenocarcinoma cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains 5-alpha Competent E. coli (High Efficiency) New England Biolabs C2987H Premo FUCCI Cell Cycle Sensor (BacMam 2.0) Life Technologies P36238 Recombinant DNA Laconic/pcDNA3.1( ) San Martin et al., 201325 Addgene: 44238 lentiCRISPR v2 Sanjana et al., 201449 Addgene: 52961 lentiCRISPR constructs with gRNA insert listed above This paper N/A Software and algorithms Fiji ImageJ N/A Gen5 v.10 Biotek N/A MATLAB R2020b Mathworks N/A Singscore R package Foroutan et al., 201850 N/A Cell Reports 43, 113612, January 23, 2024 19 ..

    Software:

    Article Title: Acid-adapted cancer cells alkalinize their cytoplasm by degrading the acid-loading membrane transporter anion exchanger 2, SLC4A2.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: VACO10MS Human: VACO4A Human: VACO4S Human: VACO5 WBL WBL WBL WBL N/A N/A N/A N/A Human: MIA Pa-Ca-2 Prof. Alessandra Fiorio, University of Lille, France N/A Human PANC-1 Prof. Anna Trauzold, University of Kiel, Germany N/A Experimental models: Organisms/strains Female athymic Nude Crl:NU(NCr)-Foxn1nu mice Charles River N/A Biological samples Matched Pair – Paraffin Tissue Section BioChain T8235090 Oligonucleotides LentiCRISPR sgSLC4A2 AAGAATCTGCGCCCTTGGCG Invitrogen N/A LentiCRISPR sgNDUFS1 TAGAATGTATGCCTACTTGG Invitrogen N/A siGENOME siControl Dharmacon D-001210-01-05 siGENOME siBNIP3 Dharmacon M-004636-01-0005 siGENOME siPEG10 Dharmacon M-032579-01-0005 siGENOME siSLC16A7 Dharmacon M-007409-01-0005 siGENOME siATP6V0A2 Dharmacon M-019950-00-0005 siGENOME siCEACAM6 Dharmacon M-015306-01-0005 siGENOME siPMP22 Dharmacon M-010616-02-0005 siGENOME siCA9 Dharmacon M-005244-02-0005 siGENOME siSLC4A2 Dharmacon M-007582-01-0005 siGENOME siSLC4A3 Dharmacon M-007583-02-0005 siGENOME siSLC26A3 Dharmacon M-007492-00-0005 siGENOME siSLC26A6 Dharmacon M-007494-01-0005 siGENOME siTFEB Dharmacon M-009798-02-0005 siGENOME ATG5 Dharmacon M-004374-04-0005 Recombinant DNA lentiCRISPR v2 Sanjana et al., 201459 Addgene: 52961 lentiCRISPR constructs with gRNA insert listed above In this paper Software and algorithms Fiji ImageJ N/A Gen5 v.10 Biotek N/A MATLAB R2020b Mathworks N/A ..

    Article Title: Dynamic IL-6R/STAT3 signaling leads to heterogeneity of metabolic phenotype in pancreatic ductal adenocarcinoma cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains 5-alpha Competent E. coli (High Efficiency) New England Biolabs C2987H Premo FUCCI Cell Cycle Sensor (BacMam 2.0) Life Technologies P36238 Recombinant DNA Laconic/pcDNA3.1( ) San Martin et al., 201325 Addgene: 44238 lentiCRISPR v2 Sanjana et al., 201449 Addgene: 52961 lentiCRISPR constructs with gRNA insert listed above This paper N/A Software and algorithms Fiji ImageJ N/A Gen5 v.10 Biotek N/A MATLAB R2020b Mathworks N/A Singscore R package Foroutan et al., 201850 N/A Cell Reports 43, 113612, January 23, 2024 19 ..

    Generated:

    Article Title: Relative expression of KLK5 to LEKTI is associated with aggressiveness of oral squamous cell carcinoma
    Article Snippet: Both synthesis of primers and oligos and sequencing of PCR products and clones were performed at the Food and Drug Administration - Center for Biologics Evaluation and Research (CBER) Facility for Biotechnology Resources at National Institutes of Health (Bethesda, MD, USA). .. The generated LentiCRISPR constructs were transfected together with the packaging plasmid psPAX2 (Addgene: 12260), and the envelope plasmid pMD2.G (Addgene: 12259) into HEK293T cells using lipofectamine 3000 as directed by the manufacturer (Thermo Fisher Scientific). ..

    Transfection:

    Article Title: Relative expression of KLK5 to LEKTI is associated with aggressiveness of oral squamous cell carcinoma
    Article Snippet: Both synthesis of primers and oligos and sequencing of PCR products and clones were performed at the Food and Drug Administration - Center for Biologics Evaluation and Research (CBER) Facility for Biotechnology Resources at National Institutes of Health (Bethesda, MD, USA). .. The generated LentiCRISPR constructs were transfected together with the packaging plasmid psPAX2 (Addgene: 12260), and the envelope plasmid pMD2.G (Addgene: 12259) into HEK293T cells using lipofectamine 3000 as directed by the manufacturer (Thermo Fisher Scientific). ..

    Plasmid Preparation:

    Article Title: Relative expression of KLK5 to LEKTI is associated with aggressiveness of oral squamous cell carcinoma
    Article Snippet: Both synthesis of primers and oligos and sequencing of PCR products and clones were performed at the Food and Drug Administration - Center for Biologics Evaluation and Research (CBER) Facility for Biotechnology Resources at National Institutes of Health (Bethesda, MD, USA). .. The generated LentiCRISPR constructs were transfected together with the packaging plasmid psPAX2 (Addgene: 12260), and the envelope plasmid pMD2.G (Addgene: 12259) into HEK293T cells using lipofectamine 3000 as directed by the manufacturer (Thermo Fisher Scientific). ..

    Virus:

    Article Title: Dynamic IL-6R/STAT3 signaling leads to heterogeneity of metabolic phenotype in pancreatic ductal adenocarcinoma cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains 5-alpha Competent E. coli (High Efficiency) New England Biolabs C2987H Premo FUCCI Cell Cycle Sensor (BacMam 2.0) Life Technologies P36238 Recombinant DNA Laconic/pcDNA3.1( ) San Martin et al., 201325 Addgene: 44238 lentiCRISPR v2 Sanjana et al., 201449 Addgene: 52961 lentiCRISPR constructs with gRNA insert listed above This paper N/A Software and algorithms Fiji ImageJ N/A Gen5 v.10 Biotek N/A MATLAB R2020b Mathworks N/A Singscore R package Foroutan et al., 201850 N/A Cell Reports 43, 113612, January 23, 2024 19 ..



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